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BM-2 Terrestrial Orchid Medium with Agar
Product ID : B142
Package size: 1 L – 10 L – 50 L
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to culture orchids. Contains 0.2 mg/L 6-benzylaminopurine (BA) & agar.
BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed Casein and L-glutamine, and 6-BA.
Description
| Solubility | Water (Partially) |
| Physical Form | Solid |
| Grams of powder to prepare 1 Liter | 27.22 |
| Other Notes | Plant Tissue Culture Tested |
| Product Number | B142 |
| Weight | 0.0000 |
Shipping and Storage
| Storage Temp. | 2 to 8 °C |
| Tariff Code | 3822.00.5090 |
Properties
Form: Powder
Appearance: White to Cream
Application: Plant Tissue Culture
Solubility: Fully Soluble in Hot Water, Partially Soluble in Cold Water
Typical Working Concentration: 27.22 g/L
Storage Temp: 2-6 °C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Same formulation as B141 with 0.2 mg/L BA; contains agar.
Unadjusted pH = 5.0 – 6.0
BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine, and 6-Benzylaminopurine (BA).
| Formula | (mg/L) |
| Boric Acid | 10 |
| Cobalt Chloride•6H2O | 0.025 |
| Cupric Sulfate•5H2O | 0.025 |
| Na2 EDTA•2H2O | 37.25 |
| Ferrous Sulfate•7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 100 |
| Manganese Sulfate•H2O | 25 |
| Molybdic Acid (Sodium Salt) •2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate•7H2O | 10 |
| Agar | 6,000 |
| 6-Benzylaminopurine (BA) | 0.2 |
| D-Biotin | 0.05 |
| Casein, Enzymatic Hydrolysate | 500 |
| Folic Acid | 0.5 |
| L-Glutamine | 100 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine•HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine•HCl |
0.5 |
Application Notes
Plant Tissue Culture Tested
Especially suited for terrestrial orchids Paphiopedilum and Phragmipedium.
References
According to “The Orchid Seedbank Project” this was originally cited by Van Waes, 1984. In vitro studie
van de kiemingsfysiologie van Westeuropese orchideeën. Thesis. Rijkuniversiteit Gent.
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Product ID : P658
Package size: 1 L – 10 L – 50 L – 100L
This medium is a modification of our Orchid Maintenance/ Replate Medium, but without banana powder. It was originally developed for the culture of Phalaenopsis stem props; it is now widely used as a seed sowing and replate medium for many epiphytic orchid species.
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